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KMID : 0360220150560081268
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Journal of the Korean Ophthalmological Society
2015 Volume.56 No. 8 p.1268 ~ p.1273
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Flow Cytometric Analysis of the Effects of Resveratrol on the Survival of Human Tennon’s Capsule Fibroblasts
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Lee See-Eun
Kim Keun-Hae
Kim Jae-Woo
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Abstract
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Purpose: Resveratrol exerts cytoprotective or cytotoxic effects according to cell type. This study was performed to evaluate the effects of resveratrol on the survival of cultured human Tenon’s capsule fibroblasts (HTFBs).
Methods: Primarily cultured HTFBs were exposed to 0, 10, or 100 μM resveratrol for 3 days. Cellular survival was assessed using the MTT assay and degree of apoptosis was analyzed with flow cytometry using annexin-V/propidium iodide double staining.
Results: Resveratrol decreased the survival of HTFBs after exposure to 10 μM (p = 0.04). In flow cytometric analysis, 10 μM resveratrol did not affect the degree of apoptosis (p = 0.89), but 100 μM resveratrol increased the degree of apoptosis (p = 0.003). Both 10 and 100 μM resveratrol did not affect the degree of necrosis (p = 0.74, 0.33).
Conclusions: Resveratrol decreased cellular survival of cultured HTFBs and induced apoptosis. Thus, resveratrol may exert antiproliferative effects on HTFBs.
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KEYWORD
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Apoptosis, Fibroblast, Flow cytometry, Resveratrol, Survival
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